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Team:UCL London/Research/MagnetoSites/Results
From 2011.igem.org
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Results
Gel photo after PCR cloning of the individual gyrase binding sites
The three bands below the 400 bp mark on the gel photo indicate successful PCR of our gyrase binding sites
Chloroquine gel displaying the difference in plasmid supercoiling between control and the three GBS
This gel clearly displays the superior property of the Mu GBS in introducing negative supercoils into a plasmid, as the bands for this plasmid travelled the farthest from the well. Although the pSC101 counterpart did manage to improve the supercoiling to a similar extent, the quality and consistency was still below that for Mu GBS. On the other hand the pBR322 GBS proved to be worse at introducing supercoils into the plasmid when compared to the control (pSB1C3 with RFP coding insert).
The effect of the presence of a Mu GBS in a plasmid on the stress response
The graph indicates a clear distinction in the level of GFP expression between cell with Spy device and a Mu GBS/Spy device ligation. So this clearly proves that the presence of the Mu bacteriophage gyrase binding site creates a difference in the level of expression of GFP from the plasmid which is conclusive towards an increased stress response.
Effect of GBS on the level and quality of supercoiling in a plasmid molecule
