Team:Paris Bettencourt/Experiments/pHyperSpank

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Team IGEM Paris 2011

pHyperSpank characterisation

The tRNA Amber diffusion system principle

The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter thant the classic one, but is also recognised by E. coli polymerases.
We cloned it first behind the amber tRNA that would induce a GFP amber on an other plasmid. Then to characterise it we cloned it behind an RFP, into TURBO cells that are LacIq to allow the promoter to be the less leacky possible.
The pictures below are the results of an IPTG induction.

Results

E. coli containing Hyperspank +RBS+RFP with and whithout IPTG at 37°C
E.coli at 37°C (trans image)
E.coli at 37°C (rfp image)
E.coli negative control at 37°C (trans image)
E.coli negative control at 37°C (rfp image)
E. coli containing Hyperspank +RBS+RFP with and whithout IPTG at 37°C
E.coli at 37°C (trans image)
E.coli at 37°C (rfp image)
E.coli negative control at 37°C (trans image)
E.coli negative control at 37°C (rfp image)

Spectrophotometry.jpg




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