Team:Paris Bettencourt/Data

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Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

See the design See the experiments

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K090403:Experience Experience] - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606039:Experience Experience] - pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039: T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
  2. [http://partsregistry.org/Part:BBa_K606036:Experience Experience] - pT7 SpoVG T7 SpoVG GFP T7terminator: T7 and GFP under a pT7 promoter to response a emission of T7.

The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606040 Main Page] - Promoter Hyperspank, BBa_K606040: IPTG Inducible promoter shortened compared to the one that was before in the registry.
  2. [http://partsregistry.org/Part:BBa_K606041 Main Page] - pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041: system for using the tRNA in a IPTG inducible system.
  3. [http://partsregistry.org/Part:BBa_K606034 Main Page] - Tyrosine amber supressor tRNA for subtilis, BBa_K606034: tRNA tyrosine amber compatible with both B. subtilis and E. coli.
  4. [http://partsregistry.org/Part:BBa_K606032 Main Page] - T7 amber, BBa_K606032: This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606037 Main Page] - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
  2. [http://partsregistry.org/Part:BBa_K606038 Main Page] - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053: promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015: standard double terminator
  3. [http://partsregistry.org/Part:BBa_K143021 Main Page] - RFP, BBa_K143021: RFP m-cherry.
  4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
  5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]

The SinOp system

The SinOp system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606046 Main Page] - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
  2. [http://partsregistry.org/Part:BBa_K606047 Main Page] - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

  1. SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]

The lambda switch system

The lambda switch system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606044 Main Page] - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 - (Imperial College, iGEM 2008)
  2. [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 - Standard double terminator.
  3. [http://partsregistry.org/Part:BBa_C0052 Main Page] - CI, BBa_B0052 - CI gene from the lambda phage.



GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143079 Experience] - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):



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