Team:Paris Bettencourt/Experiments/Methodologies/K090403J

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Team IGEM Paris 2011


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Cyrille

Control digestion of pHM3 in NcoI

CP2009.jpg

Control of the size of the insert

S75 *5; S82 *5; 104 *5
ComS *3

Baptiste, Hovannes & Ouriel

Characterisation of T7 autoloop, RFP - GFP

We characterized T7 autoloop by microscopy. Leak,


T7 autoloop RFP+ Control / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


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T7 autoloop full no terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


T7 autoloop full with terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)

YFP-tetR

We


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