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From 2011.igem.org

Contents 1 green light receptor 1.1 Inoculation 2 red light receptor 2.1 3A-assembly: Ligation and transformation 2.2 Digestion, Ligation and transfomation 3 Precipitator 3.1 PCR of GFP and plastic-binding domain

green light receptor

Inoculation

Investigators: Sophie

inoculated LB with Ccas and Ccar parts that looked good on the gel:

• S 17 (Ccar 1:1)
• S 18 (Ccar 1:3)
• S 20 (Ccas 1:3)

red light receptor

3A-assembly: Ligation and transformation

Investigators: Julia

Ligation of terminator and pycA/ho1 in pSB1C3

• L4: ho1 (S22)
• L5: pycA (S23)

...both ligated in pSB1C3 and then transformed in cells.

Comments: cells transformed with L4 have grown very good. There was only one colony of cells transformed with L5

Digestion, Ligation and transfomation

Investigators: Julia

Insert of cph8 (phytochrome) from PCR in the vector pSB1C3.

Digest of cph8:

• Enzyme 1: EcorI
• Enzyme 2: NsiI

Digest of vector pSB1T3:

• Enzyme 1: EcorI
• Enzyme 2: PstI
• Enzyme 3: DpnI

Precipitator

PCR of GFP and plastic-binding domain

Investigators: Rüdiger

Primer:

• P1: GFPxb1Iup
• P3: GFPpdbSpedw

GFP: S14 (E0040)