Team:KULeuven/Biobricks

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KULeuven iGEM 2011

Biobricks

The biobricks that we used are on the iGEM registry which can be found here. Underneath you can see several short videos in which we added different kinds of E.D Frosti to supercooled (-6°C) ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: E.D. Frosti only contains a GFP construct. When we add it to the supercooled water, it does not freeze.

The second video (INP), E.D. Frosti contains a constitutively active INP producing biobrick ( BBa_K584027). When we added this to the supercooled water, it froze immediately.