green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
Troubleshooting of the modified Lysis genes K124017
Investigators:Theo
Transformation of M66 with M48 did not work. Probably because the stock of M66 that we made was indeed another part.
So S4+S15 Nr1 is going to be Nr1 and S4+S15 Nr7 is Nr7.
M48+1 and M48+2 as well as just Nr1 and Nr2 were inoculated so that they could be sent for sequencing on Friday
Precipitator
Ligation
| Name: Rüdiger
| Date: 08.09.
|
| Continue from Date 05.09. Name Rüdiger
Experiment Digestion
|
| Project Name: Precipitator
|
Procedure
PCR tube:
total volume 20 μl
- add H2O (17 μl -X-Y-Z)
- add 2 μl Ligase Buffer 10x
- add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
- add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
- Add 1 μl T4-DNA Ligase
- Incubate 10-30 min at room temperature
- heat for 20 minutes at 80°C
- store at -20°C or directly proceed to transformation
|
| Name of part
| Ratio Insert:Vector
= 3:1 or 1:1
| Volume (μl)
|
| X insert 1
|
|
|
|
| Y insert 2
|
|
|
|
| Z vector
|
|
|
|
| H2O
|
|
|
|
Documentation:
Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.
| Name
| Vector
| Insert
|
| 4a
| C3, 05.09. digest
| 4
| 05.09. digest
|
| 8a
| C3, 05.09. digest
| 8
| 05.09. digest
|
| 9a
| C3, 05.09. digest
| 9
| 05.09. digest
|
| 10a
| C3, 05.09. digest
| 10
| 05.09. digest
|
| 4b
| C3, 05.09. digest
| 4
| 31.08. digest
|
| 8b
| C3, 05.09. digest
| 8
| 31.08. digest
|
| 9b
| C3, 05.09. digest
| 9
| 31.08. digest
|
| 10b
| C3, 05.09. digest
| 10
| 31.08. digest
|
|
NAME OF YOUR EXPERIMENT
Investigators: NAME
"
Contact 
