Team:Paris Liliane Bettencourt/Notebook/2011/07/14/

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Team IGEM Paris 2011

==Kevin==

Miniprep of lacO/tetO plasmids :

  • Centrifugation of glycerol culturs 4000 RPM - 10 min
  • Put liquid on trash and vortex it
  • Protocol of QIAprep Spin Miniprep Kit using a microcentrifuge


Contents

Mathias & Laura

Reporter BioBricks

Miniprep

-RFP (m-cherry) E1010

-Standard Terminator B0015

-GFP mut3b E0040

-T7 Terminator J18913

-pSB1C3

Digestion and gel extraction

-RFP (m-cherry) by  EcoR1 and Spe1

-Standard Terminator by EcoR1 and Xba1

-GFP mut3b by  EcoR1 and Spe1 

-T7 Terminator by EcoR1 and Xba1

-pSB1C3 by EcoR1 and Pst1

Ligation and transformation

-RFP & Standard Terminator => K606012

-GFP mut3b & T7 Terminator => K606014

-pSB1C3 & RBS => K606061

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