Materials & Method: page1

Strain:

• MC1061 : F− araD139, Δ(ara-leu)7696,galE15, galK16, Δ(lac)X74, rpsL (Strr), hsdR2 (rK−mK+), mcrA mcrB1 • DG1(Delphi genetics): mcrA Δ (mrr-hsdRMS-mcrBC, modification-, restriction-) F80lacZDM15 Δ lacX74 recA1 araD139 Δ (ara-leu)7697 galU galK rpsL endA1 nupG • TOP10 (Invitrogen) : F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- • MG1655 : F- λ- ilvG- rfb-50 rph-1 • MG1655 ΔtldD::frt-cm-frt