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Contents 1 Preparation of electrocompetent cells 1.1 Overview 1.1.1 Materials 1.1.2 Procedure

Preparation of electrocompetent cells

Overview

We use a protocol commonly used in our host lab provided by Diewertje Piebes.

Materials

Procedure

• Prepare TOP10 preculture by inoculating 3ml of LB medium with a -80d°C TOP10 glycerol stock into and shake overnight at 37°C