Team:Uppsala-Sweden/Collaboration

Team Uppsala-Sweden has helped team Freiburg with trouble-shooting PCR. Uppsala shared the primer design of some light sensor genes, as well as information on touchdown PCR with Freiburg. Notably, the primer sequence for cloning cph8 from Jeffrey Tabor's pJT122 plasmid was disclosed by Uppsala. Furthermore, PCR protocols that proved successful were shown to Freiburg. This information was also present in Freiburg's notebook (16.08).

https://2011.igem.org/Team:Freiburg/Notebook/16_August

TU Munich have had problems with assembling the red light-sensor cph8 with other DNA constructs. The ligation attempts persistently failed to work. In order to help TU Munich keep going with the progress, Uppsala sent a functional cph8 plasmid to Munich. TU Munich confirmed that they have received the plasmid and successfully transformed some cells with it. TU Munich also "checked it for correct size on gel and it looks fine". In exchange, TU Munich provided information on EnvZ/OmpR kinetics to Uppsala to assist team Uppsala with their modelling.

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