Team:Wisconsin-Madison/biosensor

From 2011.igem.org

Revision as of 11:19, 19 September 2011 by KennethXu (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)









Project >> Overview, Ethanol Sensor, Alkane Sensor, Microcompartment

Biosensors

Both the ethanol (EtOH) and n-alkane sensors rely on an arabinose inducible two plasmid system for either EtOH or n-alkane detection. The first plasmid contains genes for producing a protein controlled the pBAD promoter, which turns on transcription of the genes in the presence of arabinose. The second plasmid has the EtOH or n-alkane promoter which turns on the transcription of our RFP. The production of the RFP is then measured with a plate reader.


In the directed evolution construct, this two plasmid system is all put on a single plasmid, which the first plasmid’s parts being put on the reverse strand (5’ to 3’) of the DNA.


Learn more about biofuels, genes.