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From 2011.igem.org

• Home
• About us
  • The Team
  • Supervisors
  • Gallery
• The Project
  • Abstract
  • Introduction
  • The Plug 'n' Play
    • Assembly system
    • Customization
  • Other assembly systems
  • Achievements
  • Attributions
• Results
  • Background
  • Characterization
  • Proof of concept
    • Mammalian Cells
    • Fungi
  • Data page
  • Submitted Biobricks
  • Collaboration
• Safety
  • Safety
  • Safety rules
• Notebook
  • Lab notes
  • Protocols
• Our Partners
  • Sponsors
  • Advisors
  • Acknowledgements

Why Plug 'n' Play with DNA? We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans.

iGEM 2011 Wiki Main Page

We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi.

We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence.

We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy.

We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts requirements listed by iGEM 2011.

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