• Introduction
  • Home
  • The Team
  • Photo Gallery
• Our Project
  • Results Summary
  • Selection System
  • In-Vitro Characterization
  • In-Vivo Characterization
  • Microfluidics
  • Data
  • Attributions
• Tools
  • Gibson Assembly
  • MITOMI
• Notebook
  • Protocols
  • May
  • June
  • July
  • August
  • September
  • October
• Considerations
  • Human Practices
  • Safety
• Acknowledgements
  • Sponsors
  • Partners

T7 promoter variants

To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence.

A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter.