Team:Lyon-INSA-ENS/Realisation/Notebook/Week1

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Week 1


From Thursday the 16th of June to Friday the 17th of June 2011





Thursday

PCR and mutagenesis of rcn, csgBA, csgBAEFG

Culture of NM 522 cells for later transformations





Friday

PCR and mutagenesis of rcn, csgBA, csgBAEFG

Extraction of plasmid with NicoT gene from filter paper. Transformation of the extracted DNA in NM522 strain using TSS. Culture and selection of transformants on ampicilline plate during the week end.














ENS assystem Biomérieux INSA INSA
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