Team:WarrenCIndpls IN-HS/Notebook
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Notebook
May 4th: Bacterial and Yeast Transformation
May 11th: Gibson Assembly
May 19th: Research on Parts
Kozak Sequence- directs translation of mRNA for more efficiency and accuracy
Promoter Region- site for RNA polymerase to attach to and begin transcription; yeast has many promoters within its genome that can be used to express metal detectors
Terminator Sequence- signals the end of transcriptoin to RNA polymerase
Vector- Plasmid
ORI (Origin of Replication) Selection Markers -Ura3 is a selection marker for yeast -Ampicillin Resistance is a selection marker for bacteria based antibiotic resistance