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Team:Amsterdam
From 2011.igem.org
Revision as of 12:37, 8 September 2011 by B.Stringer (Talk | contribs)
iGEM team Amsterdam 2011
Welcome to the wiki of team iGEM Amsterdam 2011! On this site, you can find out everything about the team that represents Amsterdam in this year's edition of the iGEM competition, as well as our project: icE. coli. You will find answers to questions like...- Who are the team members?
- What are they working on?
- Who's paying for all this?
Feel free to take a look around, and be sure to contact us with any questions that remain!
Abstract
Escherichia coli's optimal growth temperature is 37°C. Its growth rate decreases drastically at lower temperatures and growth completely halts below 7°C. The aim of our project is to increase the cold tolerance of E. coli by expressing and combining several chaperone proteins that are normally found in psychrophillic bacteria. Chaperone proteins are essential in maintaining correct protein folding following changes in temperature. As such, expressing these proteins will enable enhanced growth rates at temperatures below 37°C and shift the minimal growth temperature down from 7°C, possibly even allowing growth near 0°C. Protein expression is achieved by using BioBricks, combining promoters and ribosome binding sites characterized by previous iGEM teams with newly synthesized genes that encode for psychrophile chaperones. While our subject is mainly fundamental in nature, there are various practical applications such as selection for growth on low temperatures instead of antibiotics or the optimization of biofuel production.News
26/08/2011 - First succesful assemblies!
After many weeks of trial and error, the first assemblies have succeeded! Armed with fresh construction intermediates, the race is on to make and characterize our CryoBricks!15/08/2011 - Team Amsterdam among favourites
Team Amsterdam has been pointed out as one of the teams to keep your eye on during the competition by Christian Lund, a medicine student from Denmark. He participated in last year's iGEM competition, and is now following the different teams. Read more...
Would you like to contact us? E-Mail
Follow @igemamsterdam
Would you like to help us? Donate here!
Updates
- 22/09/11 - Wikifreeze: All of our pages have been finalized!
- 08/09/11 - The BioBricks pages are undergoing a major overhaul!
- 06/09/11 - Over the last weeks all pages have received updates. Be sure to check them all!
- 10/08/11 - The Description has been updated!
- 27/07/11 - The Notebook page has been restructured!
- 26/07/11 - The Students page has been updated!
- 19/07/11 - Several meetings and lab journal entries placed onto notebook
- 13/07/11 - The Project Description and Safety pages have been updated!
- 08/07/11 - Several meetings placed onto notebook
- 06/07/11 - Added several lab journal entries
- 05/07/11 - Updated several bios and notebook
- 29/06/11 - Several meetings placed onto notebook
- 28/06/11 - Many small layout changes made
- 27/06/11 - Several meetings placed onto notebook
- 24/06/11 - Added notebook calendar
- 23/06/11 - Fixed sponsor template
- 21/06/11 - Remaining protocols added
- 20/06/11 - First protocols added
- 17/06/11 - Photos added at lab environment
- 15/06/11 - Created footer
- 15/06/11 - Created all pages
- 12/06/11 - Created first version of header
- 02/06/11 - Temporary wiki online
