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Team:Imperial College London/Notebook/week10
From 2011.igem.org
Diary
This is our diary page which records the daily activities of the team. Click on the links below to see a summary of events and activities happening each week.
Week 10: 5th September to 11th September
Monday, 5th September
Frank prepared for his talk with Tom Wakeford tomorrow. Nina and Rebekka got started on preparing the poster. Chris loaded the CPEC and discovered that none of tehm worked. However, the anti-holin PCR worked and infusion should work tomorrow. Nick did a Salkovsky assay but messed up and learned not to leave antibiotics out. Yuanwei put up the new AuxIn logo, changed the colour scheme, removed the count down on the front page and uploaded the chemotaxis animation. Ming prepared auxin concentrations and watered the plants. Nikki helped other people out with lab stuff and wrote loads of emails.
Tuesday, 6th September
Today Nina and I fixed the wiki for the Chemotaxis modeling. Yuanwei re-arranged the team page and change the skin color for the whole wiki. Frank arranged an interview with Tom Wakeford, he also prepared the workshop for radio iGEM with Chris. Apart from visiting CJ’s house for preparing the workshop, Chris did the infusion again and transformed super folded GFP. Rebbeck transformed cell with GFP cell and did another karine plate, she also did a bit work on poster. Ming helped us did some research on modeling, watered plant in the afternoon and learned the image J programme. Nick characterized the auxin part, however it failed, therefore he transformed the auxin fragment again. Nikki run a gel of RFP colonies did GFP PCR. Two of her super folded GFP PCR worked, and then she digested and purified that. She did same thing on holin as well. She also put the sequence on the wiki and emailed the seed coat expert.
Wednesday, 7th September
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Thursday, 8th September
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Friday, 9th September
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