26 (May 26, 2011 GDS)

From 2011.igem.org

Revision as of 20:44, 6 September 2011 by Pjpatel (Talk | contribs)

Created TENS

4.5 mL TE and
250uL 10% SDS
250uL 2N NaOH


-Spin 4 tubes (1.5mL) 30"
-Pour off sol'n & resuspend
-Add
300uL TENS: mix
150uL NaOH: mix
-Spin 2.5 min.
-Transfer supernatant to clean tube and add 1mL of EtOH
-Vortex and pellet DNA by centrifugation for 2.5mL
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