7 (June 7, 2011 GDS)

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-Spin 1.5 mL of overnight culture in microfuge -Aspirate all but 100uL of the supernatant and resuspend pellet by vortexing -Add 300uL of TENS and mix by inversion -Add 150uL of sodium acetate and vortex -Centrifuge for 2.5 minutes at 10K -Transfer supernatant to clean tube and add 1mL of room temp. ETOH -Mix and pellet DNA by centrifuge for 2-5 min. at 10K -Wash pellet with 70% ethanol and allow pellet to dry -Resuspend pellet in 30uL of TE w/ RNA seA -Digest 5-10uL as usual

To make TENS, add:

-4.5mL of TE

-250uL 10%SDS

-250uL 2N NaOH