Team:Osaka/week6
From 2011.igem.org
Contents |
September 4(Sun)
- Restriction digests:3-10C,1-2M
- Gel extraction of digests
- Ligation
- 03:1-9N+3-6H again
- Transformation of ligation products
- Photospectrometry measuament
September 5(Mon)
Name | IPTG(0.1M) | incubation | OD600 | A474 |
---|---|---|---|---|
1-9N | <bbpart>BBa_J22106</bbpart> | A | rec A (SOS) Promoter | |
1-2M | <bbpart>BBa_K118022</bbpart> | A | RBS | |
1-14K | <bbpart>BBa_B0040</bbpart> | A | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP | |
1-12M | <bbpart>BBa_E0240</bbpart> | A | GFP generator | |
1-1D | <bbpart>BBa_R0010</bbpart> | A | promoter(lacI regulatd) |