green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Picking clones
Investigators: Sandra
There were several clones on the cm plates. Clones were picked and incubated in LB cm medium at 37°C.
Gibson primer design
Investigators: Sophie, Sandra, Ruediger
we designed some new gibson primers for the assembly of NOT and LOV-tap in a vector because we are not sure if we can achieve this by 3A assemblies.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Ligation
| Name: Sophie
| Date: 1.9.11
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| Continue from Date: 31.08.11 Name: Sophie
Experiment: Digestion
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| Project Name:
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Procedure
PCR tube:
total volume 20 μl
- add H2O (17 μl -X-Y-Z)
- add 2 μl Ligase Buffer 10x
- add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
- add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
- Add 1 μl T4-DNA Ligase
- Incubate 10-30 min at room temperature
- heat for 20 minutes at 80°C
- store at -20°C or directly proceed to transformation
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| Name of part
| Ratio Insert:Vector
= 3:1 or 1:1
| Volume (μl)
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| X insert 1
| GFP-pbd-PCR a/b
| both
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| Y vector
| pGEX
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| H2O
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Documentation:
Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.
| Why? To get data about the plastic binding for modeling
stored in: “Minipreps, verdaut”-box
ligation-products stored in: ligation box
parts for ligation: GFP-pbd-PCR a/b, pGEX
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