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Team:Paris Liliane Bettencourt/Notebook/2011/09/03/
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Danyel
- Miniprep of S92(Pveg_spoVG_tRNA_Ter in pHM3 in MH1 cells).
- Gel extraction of S82 digested on S & P and S82 digested on E & S
- Ligation of:
- S38 in S24 (CmR)
- S38 in S82 (CmR)
- S58 in S82 (CmR)
- (with negative controls for S24 and S82)
at ratio 6:1 and 100:1
