green light receptor
3A-assembly:Digestion
Investigators: Sandra
Digestion of:
InsertI:
with EcorI and SpeI
InsertII:
with XbaI and PstI
Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hours.
blue light receptor
Stop of ligation
Investigators: Sandra
Inactivation of ligase at 80°C for 20 minutes.
Stored in the blue light box, labelled: ♥C3 Lig., and Not C3 Lig.
To do: Trafo
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Picking clones
Investigators: Sandra
Only few colonies grew on two plates. These clones were picked and incubated in LB with amp at 37°C.
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