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green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

MIniprep

Investigators: Sophie, Sandra

Miniprep of new ligated parts in pSB1C3 vector.

After miniprep samples were digested and loaded onto a gel. Not c has inserts:-)

1:2 not a has inserts too!

Digestion: 2A-assembly

Investigators: Sophie

Digestion of the PCR products Notgate, Lovtap for the 2A-assembly.

Ligation:2A-assembly

Investigators: Sandra

Ligation of the digested parts in the already digested vector pSB1C3. Ligation at room temperature over night.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017

Investigators:Theo
Sequencing of Part S66 (modified Lysis genes K124017) showed that it is a DNA fragment from our other projects, so another miniprep was done and sent for sequencing.

• Miniprep data were good:
  • Concentration = 220ng/mikrol
  • 260/280 = 2.04
  • 260/230 = 2.19

No gel was run to see if there is a band!

Precipitator

Trafo

Investigators: Julia

Trafo of ligated parts:

1. pGEX vector (Amp. resistance)
2. pGEX vector (Amp. resistance)
3. pGEX vector (Amp. resistance)
4. pSB1C3
5. pET Duet vector (Amp. resistance)
6. pET Duet vector (Amp. resistance)
7. pET Duet vector (Amp. resistance)
8. pSB1C3
9. pSB1C3
10. pSB1C3

Miniprep

Investigators: Sophie

miniprep of GFP-pbd with IPTG-inducible promoter. The pellets were green!!! So we finally got both promoter and plastic binding domain into a vector! We can make experiments with the pbd now.