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Team:Washington/Team/Members
From 2011.igem.org
Contents |
Who we are
Washington jeff johnson.png
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Chris eiben.jpg
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Washington Huber Tomas.jpg
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Washington Ivan Huang.jpg
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Washington Peixian.JPG
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Washington Denny Luan.JPG
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Wu Cindy.jpg
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Laura Austin.jpg
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Washington Justin.jpg
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Matthew Harger.JPG
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Molecular and Cellular Biology |
Ingrid.jpg
Microbiology |
 Electrical Engineering |
Galdzicki.jpg
Biomedical and Health Informatics |
Washington Siegel 2010.jpg
Biomolecular Structure and Design |
 Biochemistry |
 Electrical Engineering |
Washington Mougous.jpg
Microbiology |
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Bioengineering |
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Lab space |
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Financial support and lab space |
Wahsington Fisher Logo.jpg
Lab Materials |
Washington Arthur Friedlander.jpg
Help planning the project and offering to test our new enzymes' effectiveness against Anthrax! |
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Always available to answer our TinkerCell API questions no matter how trivial. |
Who did what
The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
The Gram Positive Therapeutic
Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.
The Gram Negative Therapeutic
Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.
Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.
New Tools
Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.
Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.
