green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Ligation
| Name:Rüdiger
| Date:19.08.
|
| Continue from Date Name
Experiment Digestion19.08. Rüdiger /Gelextraction (Theo)
|
| Project Name:Precipitator
|
Procedure
PCR tube:
total volume 20 μl
- add H2O (17 μl -X-Y-Z)
- add 2 μl Ligase Buffer 10x
- add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
- add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
- Add 1 μl T4-DNA Ligase
- Incubate 10-30 min at room temperature
- heat for 20 minutes at 80°C
- store at -20°C or directly proceed to transformation
|
| Name of part
| Ratio Insert:Vector
= 3:1 or 1:1
| Volume (μl)
|
| X insert 1
|
| 01:03
| 10
|
| Y insert 2
|
|
|
|
| Z vector
|
| 2
| 2
|
| H2O
|
|
| 5
|
Documentation:
Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.
| Trying to ligate the 3 Precipitator motifs into iGEM Vector pSB1C3
|
NAME OF YOUR EXPERIMENT
Investigators: NAME
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