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Team:Waterloo
From 2011.igem.org
In Vivo Protein Fusion Assembly Using Self Excising Ribozyme
The Ribozyme Project is the primary project for the Waterloo 2011 iGEM Team. It consists of a self-excising intron sequence which will provide a novel way to make fusion proteins without a ligation scar. For more information, please click on the links below to view the project description, safety, and up to date notebook entries.
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Project AbstractIntrons, self-excising ribozymes, can become a useful tool to create in vivo protein fusions of BioBrick parts. To make this possible, intron sequences are used to flank non-protein parts embedded in coding sequences. An intron sequence with an embedded recombination site is capable of in vivo insertion of a compatible protein fusion part. As an example, a GFP-fusion was created with an intervening lox site that is removed from the final protein using the intron to form a fully functional GFP protein. In vivo protein fusions can be applied to a larger number of modular systems to make complicated expression systems, such as synthetic antibodies or plants capable of Cry-toxin domain shuffling. | File:Waterloo team.png Your team picture |
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