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Team:Freiburg/Project
From 2011.igem.org
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Overall project
We aim to transform protein expression in bacterial systems into an elegant, fast and affordable process. By eliminating routine use of expensive materials, this novel assay will utilize sustainable laboratory equipment and widespread His-Tag technology.
We propose an expression system induced by blue, green and red light, combined with subsequent temperature controlled autolysis of E. coli. Purification of the protein of interest will be accomplished by an adaptor protein of our own design which binds the His-Tag on one side and the surface of serological pipettes on the other. Two subsequent pipetting steps for washing and purification of the cell lysate will quickly elute the product.
Our system will provide expression and purification of Polymerase and Ligase, but will be easily expandable to any His-Tagged protein needed by the modern molecular biologist.
