Team:Bilkent UNAM Turkey

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Home

Deneme Deneme2

Team Project Modelling

Deneme3 Deneme4

Safety Biobrick Parts Notebook Attributions




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      Contents

      SAFETY AND SECURITY QUESTIONS

      <p>

      1. Would the materials used in your project and/or your final product pose:

      a. Risks to the safety and health of team members or others in the lab?
      Using hazardous materials always has risk to cause trouble for team and lab members. We always
      use hoods and wearing lab coat, gloves, goggles are essential for an experiment.
      We are working on two species one of them is Chlamydomonas reinhardtii which has no dangerous
      effect on human health. Other one is Bacillus subtilis which also is not human pathogen but it
      could cause food poisoning.

      b. Risks to the safety and health of the general public if released by design or accident?
      Chlamydomonas reinhardtii is a strain of algae and it could cause algal bloom that means it
      overgrown in a water ecosystem and become poisonous for other species which share same places.
      According to a Toxic Substances Control Act report from the Environmental Protection Agency,
      Bacillus subtilis “is considered a benign organism as it does not possess traits that cause
      disease. It is not considered pathogenic or toxigenic to humans, animals, or plants. The potential
      risk associated with the use of this bacterium in fermentation facilities is low.” Its degree of
      toxicity is III and IV the lowest toxicity effect on other species.

      c. Risks to environmental quality if released by design or accident?
      We haven’t checked modified organism is more competitive than natural strains. However; Bacillus
      subtilis is well-known and commonly used model organism like Chlamydomonas reinhardtii.

      d. Risks to security through malicious misuse by individuals, groups or states?
      Our modified Chlamydomonas reinhardtii just degrade TNT and it could not use for terrorism
      activity. Those two modified organism could not have ability to damage human health. Our facility
      always has an active security system and it requires an identity card for enterance.
      Please explain your responses (whether yes or no) to these questions.
      Specifically, are any parts or devices in your project associated with (or known to cause):</i>
      - pathogenicity, infectivity, or toxicity?
      <i>No
      - threats to environmental quality?
      No
      - security concerns?
      No


      2. If your response to any of the questions above is yes:

      a. Explain how you addressed these issues in project design and while conducting laboratory work.
      Natural strain cause algal bloom and threat environmental quality so we used biological
      hazardous waste for them. And after our work is finished, we applies bleach (%5) and then it goes
      to waste.
      b. Describe and document safety, security, health and/or environmental issues as you submit your
      parts to the Registry.
      Our parts have not any safety, security and health issues. However, adding NfsI gene into algae
      could make it more competitive than normal strains. It requires checking before releasing to
      nature.


      3. Under what biosafety provisions will / do you operate?

      a. Does your institution have its own biosafety rules and if so what are they? Provide a link to
      them online if possible.
      Our institution have its own biosafety rules and it takes 19 page so we added a link below which
      leads to institution’s safety committee page and it contains two link; one of them orientation
      of our lab and other one is chemical disposal form.
      <a href="http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html">http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html</a>

      b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes,
      have you discussed your project with them? Describe any concerns or changes that were made based
      on this review.
      We have a Laboratory Safety Committee; however, we did not discuss about our project because our
      experiment contains standard cloning procedure, which has always done in the lab.

      c. Will / did you receive any biosafety and/or lab training before beginning your project? If
      so, describe this training.
      Our institution have an exam about biosafety, we passed it. It consists of orientation which
      mentioned above.

      d. Does your country have national biosafety regulations or guidelines? If so, provide a link to
      them online if possible.
      <a href="http://www.unep.org/biosafety/files/TRNBFrep.pdf">http://www.unep.org/biosafety/files/TRNBFrep.pdf</a>

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      Home Team Project Modelling Safety



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        SAFETY AND SECURITY QUESTIONS

        <p>

        1. Would the materials used in your project and/or your final product pose:

        a. Risks to the safety and health of team members or others in the lab?
        Using hazardous materials always has risk to cause trouble for team and lab members. We always
        use hoods and wearing lab coat, gloves, goggles are essential for an experiment.
        We are working on two species one of them is Chlamydomonas reinhardtii which has no dangerous
        effect on human health. Other one is Bacillus subtilis which also is not human pathogen but it
        could cause food poisoning.

        b. Risks to the safety and health of the general public if released by design or accident?
        Chlamydomonas reinhardtii is a strain of algae and it could cause algal bloom that means it
        overgrown in a water ecosystem and become poisonous for other species which share same places.
        According to a Toxic Substances Control Act report from the Environmental Protection Agency,
        Bacillus subtilis “is considered a benign organism as it does not possess traits that cause
        disease. It is not considered pathogenic or toxigenic to humans, animals, or plants. The potential
        risk associated with the use of this bacterium in fermentation facilities is low.” Its degree of
        toxicity is III and IV the lowest toxicity effect on other species.

        c. Risks to environmental quality if released by design or accident?
        We haven’t checked modified organism is more competitive than natural strains. However; Bacillus
        subtilis is well-known and commonly used model organism like Chlamydomonas reinhardtii.

        d. Risks to security through malicious misuse by individuals, groups or states?
        Our modified Chlamydomonas reinhardtii just degrade TNT and it could not use for terrorism
        activity. Those two modified organism could not have ability to damage human health. Our facility
        always has an active security system and it requires an identity card for enterance.
        Please explain your responses (whether yes or no) to these questions.
        Specifically, are any parts or devices in your project associated with (or known to cause):</i>
        - pathogenicity, infectivity, or toxicity?
        <i>No
        - threats to environmental quality?
        No
        - security concerns?
        No


        2. If your response to any of the questions above is yes:

        a. Explain how you addressed these issues in project design and while conducting laboratory work.
        Natural strain cause algal bloom and threat environmental quality so we used biological
        hazardous waste for them. And after our work is finished, we applies bleach (%5) and then it goes
        to waste.
        b. Describe and document safety, security, health and/or environmental issues as you submit your
        parts to the Registry.
        Our parts have not any safety, security and health issues. However, adding NfsI gene into algae
        could make it more competitive than normal strains. It requires checking before releasing to
        nature.


        3. Under what biosafety provisions will / do you operate?

        a. Does your institution have its own biosafety rules and if so what are they? Provide a link to
        them online if possible.
        Our institution have its own biosafety rules and it takes 19 page so we added a link below which
        leads to institution’s safety committee page and it contains two link; one of them orientation
        of our lab and other one is chemical disposal form.
        <a href="http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html">http://unam.bilkent.edu.tr/UNAM%20Laboratory%20Safety.html</a>

        b. Does your institution have an Institutional Biosafety Committee or equivalent group? If yes,
        have you discussed your project with them? Describe any concerns or changes that were made based
        on this review.
        We have a Laboratory Safety Committee; however, we did not discuss about our project because our
        experiment contains standard cloning procedure, which has always done in the lab.

        c. Will / did you receive any biosafety and/or lab training before beginning your project? If
        so, describe this training.
        Our institution have an exam about biosafety, we passed it. It consists of orientation which
        mentioned above.

        d. Does your country have national biosafety regulations or guidelines? If so, provide a link to
        them online if possible.
        <a href="http://www.unep.org/biosafety/files/TRNBFrep.pdf">http://www.unep.org/biosafety/files/TRNBFrep.pdf</a>



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