Team:UC Davis/Notebook/Week 10

From 2011.igem.org

Revision as of 21:47, 16 August 2011 by NCsicsery (Talk | contribs)

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

--Monday 8/15/11--


We got our sequences from last week in and checked them to make sure they looked good. Not all of the parts were correct, but we are about a day or two from being done with construction! Here is a table of which parts are completed and which parts we need to complete in order to finish construction.

LacIc1TetR
Repressor Screening*R0010+E0240+I13453+B0034R0051+E0240+I13453+B0034 *R0040+E0240+I13453+B0034
Promoter ScreeningE0240+I13453+B0034+C0012E0240+I13453+B0034+C0051E0240+I13453+B0034+C0040
Wild Type Controls*R0010+E0240+I13453+B0034+C0012 R0051+E0240+I13453+B0034+C0051*R0040E0240+I13453+B0034+C0040


' LacI c1TetR
Repressor Screening R0010+E0240+I13453+B0034 R0051+E0240+I13453+B0034 R0040+E0240+I13453+B0034
Promoter Screening E0240+I13453+B0034+C0012 E0240+I13453+B0034+C0051 E0240+I13453+B0034+C0040
Wild Type Controls R0010+E0240+I13453+B0034+C0012 R0051+E0240+I13453+B0034+C0051 R0040E0240+I13453+B0034+C0040

In order to finish our construction, we are going to cut out R0010 and R0040 as inserts and put them in front of the parts needed. Tomorrow We will transform and hopefully have the finished construct by Wednesday.

--Tuesday 8/16/11--

Ligated and transformed yesterday's digestion.

Retrieved from "http://2011.igem.org/Team:UC_Davis/Notebook/Week_10"