Team:Freiburg/Notebook/16 August

From 2011.igem.org

Revision as of 13:54, 16 August 2011 by Juliimapril (Talk | contribs)


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Picking clones

Investigators: Sandra

Picking clones of transformed cells with LovTAp, NotGate, pSB1T3.

red light receptor

continuing with PCR of cph8

Investigators:Julia
2µl of the PCR reaction from 15th Aug. were loaded onto a gel.
But there was no band. Jakob is going to repeat the PCR.

3a-assembly with promotor+ribosome binding site infront of pcyA and ho1

Investigators:Julia Digestion

  • PR1 (pSB1C3)
  • PR2 (pSB1C3)
  • pcyA (pSB1T3)
  1. pcyATd (pSB1T3)
  2. pcyATb (pSB1T3)
  • L23 (pSB1T3)
  • vector (pSB1K3)


Amount of DNA and H20:

Sample 500(ng)/DNA concentration (ng/μl) H20 μl
pcyATd 2.8 35.2
pcyaTb 2.9 35.1
PR2 7.2 30.8
PR1 6.1 31.9
S22a 2,5 35.5
pSB1K3 20 18


Enzymes necessary for digestion:

PR1/PR2 pcyA/S22a vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 SpeI PstI PstI


-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C


Ligation

Ligation of:

PcyATd S22a (ho1)
PR1 1 pTd 1 pTb
PR2 2 pTd 2 pTb

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/16_August"