Team:Cornell/Week 11

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Results | Protocol | Notebook | Parts Submitted

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August 14th - August 20th

Sunday, August 14

Afternoon lab work done by: Charlie Chung

  • Picked three colonies from the two (GFP + Avi-Tagged pZE12 backbone) plates
- GFP + Avi + BB (1) #1, 2, 3
- GFP + Avi + BB (2) #1, 2, 3
  • Incubating overnight in 37°C shaker of Room 304
  • Re-picked three colonies from the (RFP + Avi-Tagged pZE12 backbone) plate in order to retry subculturing, induction, and cell lysis
- RFP + Avi + BB #7, 8, 9
  • Incubating overnight in 37°C shaker of Room 304

Monday, August 15

Afternoon lab work done by: Youjin Cho and Maneesh Gupta

Obejective
  • Miniprep GFP samples that were cultured overnight and send them off to sequencing.
  • Set-up the PCR for vioB using vio operon.
Miniprep & Sequencing
  • Miniprepped the GFP samples using standard Qiagen Miniprep protocol.
GFP+Avitag+pZE12 backbone (1)- 1,2,3
GFP+Avitag+pZE12 backbone (2)- 1,2,3
  • Set-up the samples for sequencing using the reverse primer.
Order number: 10254977
PCR reaction
  • Set-up the vioB PCR using the vio operon (=20.3ng/μL).
  • As Didi suggested, used the melting temperature of 53°C and annealing time for 3:30 minutes.

Tuesday, August 16

Wednesday, August 17

Thursday, August 18

Friday, August 19

Saturday, August 20