green light receptor
Results Transformation
We dont see any colonies
- To-do: do quickchange again.
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
new 3A assenbly with Amp Vector
Investigators: Sophie
3A assembly
The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (PR4 and PR6) with different RBS binding strenght.
Name of the samples:
ε5 (GFP-pbd),
pSB1A3,
PR4,
PR6.
stored in "Minipreps, verdaut"-box
Ligation
I ligated ε5 (GFP-pbd) to PR4 or PR6 and to the Amp-Vector. Therefore I once took the ratio 3:1 insert to vector and once the ration 1:1 insert to vector.
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