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Team:KULeuven/NotebookDaily
From 2011.igem.org
tuesday 5th of July
Cell culture medium and agar plates containing antibiotics (ampicillin, kanamycin, tetracyclin and chloramphenicol) were prepared. A precul- ture of competent cells was made (protocol competent cells).
wednesday 6th of July
Competent cells were prepared according to the CaCl2 method.
thursday 7th of July
Biobricks were transformed in competent cells. List of biobricks:
1. pLux-CI promotor BBa K091107
2. pLac/Mnt Hybrid Promoter BBa K091104
3. cspA Promoter BBa K328001
4. luxI Bba C0061
5. luxR Bba C0062
6. pconst-RBS-MelA Bb K193602
7. CrtEBI Bba K274100
8. CI repressor BBa R0051
9. Mnt repressor BBa C0072
10. Colicin E2 operon without SOS promotor BBa K131009
11. ribolock3c BBa J23031
12. ribokey3c Bba J23008
13. ribosome binding site BBa B0034
14. terminator BBa B0015
15. OmpA Bba K103006
16. TEV cleavage site Bba K128002
17. GFP Generator BBa E0240
These transformed cells were grown on agar plates containing the cor- rect antibiotic for selection.
friday 8th of July
Competent cells were prepared according to the CaCl2 method.
friday 22th of July
After reformatting the laptop, it took windows 2 hours to update! Installation of maya, an amazing 3D-visualising program, provided by iGEM sponsor the mathworks. We checked the price and normally it costs $3,251! Time to watch tutorials... But first an evening dinner with our team.
monday 25th of July
Importation of 3D- structures of icenucleating protein and anti-freezeprotein in maya: check! Found some cool video made in maya. So, it should be possible to make one, but how long will it takes us?
tuesday 26th of July
Working in simbiology.
wednesday 27th of July
We made a simulation for a hybrid promotor lac_lux (BBa_R091100) which is almost synthesized in the wet lab coupled to GFP. Values of parameters are estimated, a recurring theme in kinetic modelling?
thursday 28th of July
Today we brainstormed on how we could make a molecular movie about are project.
