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Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme
From 2011.igem.org
Ozyme digestion
This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.
Procedure
1. In an eppendorf tube, add the following solutions in any order :
-11µL water
-10µL DNA
-2.5µL NE Buffer 2
-0.5µL BSA X50
2. Add 0.5µL of each one of the desired enzymes.
3. Incubate at 37°C for 10mn
4. Inactivate the enzymes by a 20mn incubation at 80°C
