From Monday the 27th of June to Friday the 1st of July 2011

Monday

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Tuesday

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Culture of NM522 cells for later transformations and Curli for extraction.

Wednesday

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Miniprep, digestion and electrophoresis of the Curli plasmid.

Thursday

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CaCl2 chemical transformation of some iGEM kit distribution DNA :

Plate 1 :
18A (constitutive promoter, Amp )
2M ( strong RBS, Amp )
5L ( weak RBS, Amp )
22M ( RBS+YFP, Amp)

Plate 2 :
24E (YFP, Amp + Kan )
2L (GFP, Amp )

The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization.

Friday

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Miniprep using the QuickPure kit of the previous 6 iGEM parts.

Digestion with :
S + P : 18A, 2M, 5L
X + P : 22M, 2L, 24E

Electrophoresis of the digested plasmids versus the non digested.
All the strains had the correct plasmid : they were plated on LB + amp medium.