Team:Caltech/Week 6

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Caltech iGEM 2011

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July 18

Colony PCR of positive Gibson plates from Friday
Gel of short PCR parts to confirm they are being amplified
Pulsed field gel with a larger amount of soil sample DNA
Retransform pSB3K5 into Xl-10s

Results

July 19

PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control

Results

pSB3K5 plate had 0 colonies.
PFGE of soil-extracted DNA shows a smear; will do gel extraction of ~40kbp sequences for fosmid kit.

Gibson PCR concentrations

Part	Concentration (ng/ul)
R0010 for pNT001	86.4
B0014 for pNT001	128.3
B0014 for pNT003	89.1
promoter for GFP Gibson positive control	51.9
GFP for positive control	130.4
terminator for positive control	82.5