"

From 2011.igem.org

Ligation of promotor, RBS in vector

Investigators: Sophie, Theo, Sandra

Comments:For ligation digested samples were used from yesterday.

Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol.

PR1: S1 with S4 (660bp)
PR2: S1 with S5 (1000bp)
PR3: S1 with S6 (426bp)
PR4: S2 with S4 (233bp)
PR5: S2 with S5 (940bp)
PR6: S2 with S6 (981bp)
PR7: S3 with S4
PR8: S3 with S5
PR9: S3 with S6

Name for each construct:

	sample	name
Promotor	BBa_J23104	S1
Promotor	BBa_J23110	S2
Promotor	BBa_J23116	S3
RBS	BBa_B0034	S4
RBS	BBa_B0032	S5
RBS	BBa_B0031	S6
Vector	pSB1C3	S7