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From 2011.igem.org

Contents 1 21. Labday 2 3A assembly:Digestion, Ligation and Transformation for the receptor 3 PCR of GFP fused to plastic-binding domain 4 Digestion of minipreps for the green light receptor

21. Labday

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3A assembly:Digestion, Ligation and Transformation for the receptor

Investigators: Julia

Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).

Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI

Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI

ligated vector was then transformed in cells and plated out on chloramphenicol plates.

Comments: The terminator between ho1 and pycA was missing. The digestion was repeated on june, 29.

PCR of GFP fused to plastic-binding domain

Investigators: Sophie, Rüdiger

S 14: GFP

Primer:

• GFPxb1Iup (P1)
  
• GFPpbdSpedw (P3)

Comments: PCR did not work, because of too high concentration of primers. PCR was repeated on june, 30

Digestion of minipreps for the green light receptor

Investigators: Sophie

Digestion of minipreps:

• S 17 (Ccar 1:1)
• S 18 (Ccar 1:3)
• S 19 (Ccas 1:1)
• S 20 (Ccas 1:3)
• S 21 (artificial terminator)

Enzyme 1: XbaI Enzyme 2: PstI

two gels were loaded.

Comments: S17, S18 and S20 were ok. S21 was not digested.