Team:Caltech/Week 5

July 11

Miniprep pSB4A5
Run PCR to amplify [http://partsregistry.org/Part:BBa_R0010 R0010], [http://partsregistry.org/Part:BBa_K123003 K123003], [http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_PSB4A5 PSB4A5]
Run gel of PCR products
Redo PCR of [http://partsregistry.org/Part:BBa_B0014 B0014]

Results

The PCR of parts for pNT003 was successful except for the terminator (B0014), as there is no band in that lane.

1 ladder, 2 R0010, 3 K123003, 4 B0014, 5 pSB4A5

July 12

Transformed competent cells with pSB3C5. Ran PCR of parts for pNT001 and pNT002 Gibson assembly. Started pulsed field gel electrophoresis of some of our LA River DNA obtained by the Mo Bio kits in week 2.

Results

All of the parts for pNT001, pNT002 and B0014 were PCR'd with primers for Gibson assembly. All lanes except for lanes 4 and 5 had single bands, indicating that the parts were successfully amplified.

from left to right: 1 ladder, 2 R0010, 3 K123000, 4 B0014 for pNT002, 5 R0040, 6 K123001, 7 B0014 for pNT003, 8 B0014 for pNT001