Team:Freiburg/Notebook/15 June

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??.Labday

PCR for Ccas and CcaR

Investigators: Julia

Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein
Ccar: cognate response regulator

Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6

32,5 µl H2O
2µl Primer fw
2µl Primer rev
1µl DNA ( synechocystis from Hess-Lab)
1µl dNTPs
0,5 µl Phusion