Copenhagen/1 July 2011

From 2011.igem.org

Friday

Wuuuhuuuuu! We have recieved our human cytochrome p450s with the mail today. What a relief. We are very grateful and send many happy thoughts to the sweet professor Guengerich who supplied them.

Lab Work

• We did a Mini prep of what we hope to be a plasmid containing CYP79B1
• We cut the (hopefully) purified CYP79A2 and CYP79B1 with restrictionsenzymes Pst1 and Xba1 to confirm that our mutations have destroyed these restrictionsites.
• We transformed competent cells with the mutants we made yesterday (with new and improved math)
• We made a lot of LB plates for future use.

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