Team:NTNU Trondheim/Protocols

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Protocols / Recipes used in the lab

LB Broth

  • Bacto-tryptone: 10 g/L
  • Yeast extract: 5 g/L
  • NaCl 5 g/L

Adjust pH to 7,4 with NaOH, autoclave.

LA

  • Dissolve LB
  • Add agar, 15 g/L

Adjust pH to 7,4 with NaOH, autoclave.

SOC:

  • Bacto-tryptone: 20 g/L
  • Yeast extract: 5 g/L
  • NaCl 0,5 g/L
  • 10 mL 250 mM KCl (25mM) → 0,186 g / 10mL H20
  • 5 mL 2M MgCl2 (10mM) → 0,952 g / 5mL






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