Team:NCTU Formosa

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Project
- Introduction
- RNA Thermometer
  - Design
  - Data
  - Modeling
- CI promoter
  - Design
  - Data
  - Modeling
- Carotenoid synthesis pathway
  - Design
  - Data
- Butanol pathway
  - Design
  - Data
- Violacein pathway
  - Design
  - Data
Measurements
Parts
Safety
Human Practice
Attribution
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Concept

Achievements

	Register our team.
	Complete the judging form.
	Set up a team wiki.
	Prepared a poster and a presentation
	Submit 46 new BioBrick parts.
	Design several new BioBricks, including Carotenoid synthesis Pathway, Violacein biosynthesis pathways, and Butanol synthesis pathway.The Butanol synthesis pathway can produce Biofuel!
	Most of our BioBricks work as expected.
	Characterize several BioBricks (BBa_K118008, BBa_K274004).
	Our favourites are BBa_K539691, BBa_K539151, BBa_K539281, BBa_K539461, andBBa_K539742.
	Incorporated Human Practices and modeling into our design.
	Designed Pathway commander, a novel concept to to control a pathway to stop at any intermediate state.

Abstract

As one of the main challenges of in vivo experiments is controlling the flux through a synthetic metabolic pathway, it’s design lies in selecting well- matched genetic components that when coupled, can reliably produce the desired behavior. Although model equations can calculate parameter values, a challenge still remains in selecting the bio-bricks that can reliably implement a desired cellular function with quantitative values. In previous studies, synthetic biologists have created numerous synthetic circuits; each generating different protein expression levels in order to test performance in reliability and consistency, but this process is both tedious and time consuming. To overcome this problem, our team designed a novel circuit design: method_ Pathway Commander. By this design method, we construct a single version of a synthetic metabolic pathway circuit that can use culture temperature shifts to control the expression levels of a series of metabolic proteins at the precise times. We have implemented the Pathway_Commander design in (1) Carotenoid synthesis Pathway, (2) Violacein biosynthesis pathways and (3) Butanol synthesis pathway in E. coli. This circuit design utilizes a temperature controlled system that gives precision control over metabolic protein expression which amounts to optimize the synthesis that can maximize synthesis of a given compound or drug.

Human Practice Members Attribution

Project Parts Measurement

Notebook Safety