The Composition Of Media

LB Medium

Yeast extact 5g

Peptone 10g

NaCl 10g

Agar 1-2%

Distilled water 1000ml pH 7.0

Boil the mixture in autoclave at 121℃ for 30min

Range of applicationb: Escherichia.coli

MRS Medium

Peptone 10g

Beef extract 10g

Yeast extact 5g

K₂HPO₄ 2g

Tween 80 1ml

Diamine citrate 2g

Na-acetate 5g

MgSO₄.7H₂O 0.5g

MnSO₄.H₂O 0.25g

Glucose 20g

Agar 1-2%

Distilled water 1000ml Adjust pH to 6.4-6.8

Boil the mixture in autoclave at 115℃ for 20min

Range of application: Lactobacillus bulgaricus

2.5% glycocoll SMRS is MRS supplemented with 2.5% glycocoll 0.5mol/L saccharose, 20mmol/L MgCl₂,20mmol/L CaCl₂.

SMRSMC is MRS supplemented with 0.5mol/L saccharose, 20mmol/L MgCl₂,20mmol/L CaCl₂.

Hogg-Jago glucose broth(HJG) medium

Competent Cell and Electroporation

The competent cell of Lactobacillus bulgaricus

The competent cell of Streptococcus thermophilus

PCR Reaction

Agarose Gel Electrophoresis

Gel Purification of DNA (Solarbio QIAquick Gel Extraction Kit)