Team:NYMU-Taipei/results/optomagnetic-design1

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• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

Six Constructs and Experimental Results

We now follow the steps we construct our design to examine and illustrate what we have done so far. The total parts we construct and their detailed information will be performed and recorded in our part registry.

Fig. 1:This is the total constructs of our optomagnetic design, the notation for each columns with different colors can be seen in the following paragraphs.

The Beginning

As soon as we have the AMB-1 bacteria colony, we do the AMB-1 colony PCR to get the Mms13's DNA sequences as our parts to do the following steps.

The correcterized gel electrophoresis result is shown below.(See Figure 2) The detail of Mms13's information, please link [http://partsregistry.org/wiki/index.php?title=Part:BBa_K624005].

Fig. 2:The correcterized gel electrophoresis result of Mms13.

Construct Mms13

Then, after we have the fundamental material of Mms13, we did the next step of our construct.(See Figure 3)

Fig. 3:We used several steps of recombinant PCR to assemble (1)mms13-rLuc； (2)YN-mms13-rLuc； (3)Y-mms13-rLuc, why we chose recombinant PCR is because of two reasons: (1) the less time it took and (2)we found better precision in recombinant PCR than in ligation process.

The correcterized and checked results shown in Figure 4.

Fig. 4:Checked YFP-Mms13-rluc part.

For parts mms13-rLuc, link[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624007]; YN-mms13-rLuc, link[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624008]; EYFP-mms13-rLuc fusion, link[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624006]