Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin</li>
Literature survey on expression of Proteorhodopsin in E.coli</li></br></br>
Week 2
Minimal media characterization of DH5-alpha</li>
Modelling survival of PR transformed E.coli cells v/s wild type E.coli</li>
Literature survey on increase in recombinant protein expression in PR transformed cells</li></br></br>
Week 3
Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.</li>
Designing the incubator for experimental studies with Proteorhodopsin</li>
Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide</li>
Week 4
Standardizing the protocol for digestion of pKJ900</li>
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