Team:TzuChiU Formosa/Notebook/photopaper

Photopaper

Meeting Minutes

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2011.02.24
Discussion:

• Team organization
  
• Brain storming
  • paper made by bacteria with add-ons such as colors, fragrance, etc.
  • "light up" the plants for replacing lamp posts.

2011.03.04
Discussion:

• Team advisory
• Brain storming
  • Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
  • information exchange with iGEM 2009 Cambridge team

2011.03.14
Discussion:

• Task Allocation
• Brain storming
  • Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
  • Eco-friendly warmer - biotic thermal pad

2011.03.23
Discussion:

• • Project : paperia
  • Option 1 : Culture bacteria which has pigment gene
  • Option 2 : Cellulose-producing bacteria secrete pigment into the medium

2011.03.24
Discussion:

• Exp. procedure:
  • cloning of cellulose gene’s CDS
  • the product should operate within E. coli.

2011.06.22
Discussion:

• Due to some unforseen reason, the team decided to change their project.
• New project: Biojenny

　　　　　　　　　-economical and humane way to produce paper in large quantities.
　　　　　　　　　-yeast to be our host

2011.07.01
Discussion:

• Freeze > grin > genome DNA isolation > Cloning = silk protein gene

2011.07.09
Discussion:

• the connections between 3 silk proteins : Fibl Fibh P25
• major proteins : H-chain, L-chain, P25

2011.07.15
Discussion:

• Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
• However it would be modified to be more innovative and creative.

2011.07.18
Discussion:

• Latest project : Photo paper
• cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.

2011.07.23
Discussion:

• system modification to overcome the problems arises during preliminary round
• Biobricks from Tokyo 2010 team will be utilized
  • regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria

2011.09.15

Genome miniprep
Gluconacetobacter hansenii

2011.09.18

Gel/PCR DNA extraction
Gluconacetobacter hansenii

Protocols

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