Team:TzuChiU Formosa/Notebook/photopaper

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Photopaper

Meeting Minutes

2011.02.24
Discussion:

Team organization
Brain storming
- paper made by bacteria with add-ons such as colors, fragrance, etc.
- "light up" the plants for replacing lamp posts.

2011.03.04
Discussion:

Team advisory
Brain storming
- Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
- information exchange with iGEM 2009 Cambridge team

2011.03.14
Discussion:

Task Allocation
Brain storming
- Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
- Eco-friendly warmer - biotic thermal pad

2011.03.23
Discussion:

- Project : paperia
- Option 1 : Culture bacteria which has pigment gene
- Option 2 : Cellulose-producing bacteria secrete pigment into the medium

2011.03.24
Discussion:

Exp. procedure:
- cloning of cellulose gene’s CDS
- the product should operate within E. coli.

2011.06.22
Discussion:

　　　　　　　　　-economical and humane way to produce paper in large quantities.
　　　　　　　　　-yeast to be our host

2011.07.01
Discussion:

2011.07.09
Discussion:

2011.07.15
Discussion:

Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
However it would be modified to be more innovative and creative.

2011.07.18
Discussion:

Latest project : Photo paper
cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.

2011.07.23
Discussion:

system modification to overcome the problems arises during preliminary round
Biobricks from Tokyo 2010 team will be utilized
- regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria

2011.09.15

Genome miniprep
Gluconacetobacter hansenii

2011.09.18

Gel/PCR DNA extraction
Gluconacetobacter hansenii

Protocols

caption

2011.10.01

modelling test